EuMMCR unit is one of the ES cell distribution units of the Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) in Munich, Germany. The EuMMCR unit distributes material arising within the EUCOMM consortium. Currently targeting vectors and ES cells generated in the EUCOMM program are distributed by the EuMMCR unit.
Upon user request we grow targeting vectors from glycerol stocks and prepare vector DNA. The identity of the vector is then verified by restriction mapping. DNA of vectors that passed this EuMMCR quality control are then sent to the requestor.
Upon user request we thaw, expand and re-freeze several aliquots of a desired ES cell clone. Our standard controls include a PCR based assay to ensure that ES cells are mycoplasma free and a genotype verification using a short PCR strategy. ES cells, which have passed both EuMMCR quality controls will be sent to the requestor.Upon additional request the EuMMCR unit also develops a genotyping PCR, which can be used to genotype the chimeric mice that may be generated using those ES cell clones.